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Table 1 Bacterial strains and plasmids used in this work

From: Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis

Strain/plasmid Relevant characteristics Source
Bacterial strains
E. coli TG1 supE, hsd, Δ5, thi, Δ (lac-proAB), F'(traD36 proAB-lacZΔM15) [50]
L. lactis NZ9000 L. lactis subsp. cremoris (derivative strain of MG1363, carrying nisRK genes on the chromosome) [51]
Plasmids
pMal-GroEL pMal expression vector carrying the B. abortus groEL gene [17]
pCYT:Nuc pWV01/Cmr; expression vector containing the fusion rbsUsp 45::nucB, under the control of P nisA [34]
pSEC:Nuc pWV01/Cmr; expression vector containing the fusion rbsUsp 45::spUsp 45::nucB, under the control of P nisA [34]
pSEC:LEISS:Nuc pWV01/Cmr; expression vector containing the fusion rbsUsp 45::spUsp 45::LEISS::nucB, under the control of P nisA [33]
pCYT:groEL pWV01/Cmr; plasmid containing the fusion rbsUsp 45::groEL, under the control of P nisA This work
pSEC:groEL pWV01/Cmr; plasmid containing the fusion rbsUsp 45::spUsp 45::groEL, under the control of P nisA This work
pSEC:LEISS:groEL pWV01/Cmr; plasmid containing the fusion rbsUsp 45::spUsp 45::LEISS::groEL, under the control of P nisA This work