Figure 1
From: Heterologous expression of Brucella abortus GroEL heat-shock protein in Lactococcus lactis
Schematic representation of expression cassettes for controlled and targeted GroEL production in L. lactis. For details of plasmid constructions, see the text and Table 1. P nisA : nisin-inducible promoter; rbsUsp 45: ribosome binding site of Usp45 gene; spUsp 45: DNA sequence encoding the signal peptide of Usp45 gene; LEISS: DNA sequence encoding the LEISSTCDA synthetic propeptide; groEL: B. abortus groEL coding sequence (not to scale).