Table 3 Primers for synthesis of in vitro RNA standards. The T7 promoter sequence was added to the 5' side of all primer 1 sequences.
From: Transient increase of ATP as a response to temperature up-shift in Escherichia coli
Name | Sequence (5'-3') | Position |
|---|---|---|
dnaK | ||
Primer 1 | CTCTTGTGTAGCGATTATGG | 39 |
Primer 2 | GTTGTTTGCAGAAGCATCAG | 1860c |
Ibp | ||
Primer 1 | CCGCTTTACCGTTCTGCTA | 22 (ibpA) |
Primer 2 | CTATTTAACGCGGGACGTTC | 425c (ibpB) |
Lon | ||
Primer 1 | ATGAATCCTGAGCGTTCTGA | 1 |
Primer 2 | CACAACCTGCATACCAGAC | 2342c |
T7 promoter | CTAATACGACTCACTATAGGGAGA |