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Figure 3 | Microbial Cell Factories

Figure 3

From: Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors

Figure 3

Comparison of the protease efficiency of TEV and C3. A) The Trx-EYFP fusion protein recovered using the pETM20 and pETM22 vectors was digested in the presence of 1 μg (TEV) and 0.2 μg (C3) protease for 100 μg of fusion protein. B) The Trx-EYFP constructs resuspended in different buffers were digested at 30°C for 3 h in the presence of 1:100 (TEV) and 1:500 (3C) diluted proteases.

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Microbial Cell Factories

ISSN: 1475-2859

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