Table 1 Bacterial toxins and their derivatives successfully expressed in P. pastoris. The bacterial toxin and the species it is originating from are given, along with brief notes on the specifics of the reported recombinant expression strategies.
Bacterial toxin (species) | Remarks (expression culture type) [reference] | Final Yield§ |
|---|---|---|
TeNT(H C ) (Clostridium tetani) | intracellular expression†of a synthetic‡ gene encoding the tetanus toxin fragment C (B) [18] | 12 g·l-1 culture* |
BoNTA(H C ) (Clostridium botulinum) | intracellular expression†of a synthetic gene‡ encoding the heavy fragment C of the botulinum neurotoxin serotype A [BoNTA(HC)] (B) [19, 22-25] | 770 mg·l-1 culture |
BoNTB(H C ) (Clostridium botulinum) | intracellular expression†of a synthetic gene‡ encoding the heavy fragment C of the botulinum neurotoxin serotype B [BoNTB(HC)] (B) [1, 20, 24] | 390 mg·kg-1 cells |
BoNTC 1 (H C ) (Clostridium botulinum) | intracellular expression†of a synthetic gene‡ encoding the heavy fragment C of the botulinum neurotoxin serotype C1 [BoNTC1(HC)] (B) [25] | 200–500 mg·kg-1 cells |
BoNTE(H C ) (Clostridium botulinum) | intracellular expression of a synthetic gene‡ encoding the heavy fragment C of the botulinum neurotoxin serotype E [BoNTE(HC)] (B) [25] | 200–500 mg·kg-1 cells |
BoNTF(H C ) (Clostridium botulinum) | intracellular expression of a synthetic gene‡ encoding the heavy fragment C of the botulinum neurotoxin serotype F [BoNTF(HC)] (B) [21, 26] | 240 mg·kg-1 cells |
DT (Corynebacterium diphtheriae) | secreted expression of a synthetic gene‡ encoding the truncated diphtheria toxin (DT) fused to a bivalent antibody fragment (B) [30-33] | 120 mg·l-1 culture* |
BSP1 and BSP2 (Bacillus sphaericus) | intracellular co-expression†of synthetic genes‡ encoding the mosquitocidal B. sphaericus polypeptides 1 and 2 (BSP1 and 2) (SF) [44] | <30% tcp* |
Cry2 (Bacillus thuringiensis) | intracellular expression of Cry2 using the native bacterial DNA sequence (SF) [43] | N.D. |
Cyt2Aa1 (Bacillus thuringiensi s) | intracellular expression of a synthetic gene‡ encoding Cyt2Aa1 (SF) [34] | ~1 mg·l-1 culture* |
Cyt2Aa1 (Bacillus thuringiensis) | synthetic gene‡ encoding Cyt2Aa1 fused to a human scFv; secretory targeting resulted in ER-retention of the recombinant product (SF) [35] | 10 mg·l-1 culture |
Ace (Vibrio cholerae) | secreted expression of the accessory cholera enterotoxin (Ace) using the native bacterial DNA sequence (SF) [28] | 7 mg·l-1 culture* |
Cef (Vibrio cholerae) | secreted expression of Chinese hamster ovary (CHO) cell-elongating factor (Cef) using the native bacterial DNA sequence (SF) [29] | N.D. |
CTB (Vibrio cholerae) | secreted co-expression of the cholera toxin subunit B (CTB) and CTB-viral antigen fusion protein using the native bacterial DNA sequence (SF) [4] | N.D. |
LTB (Escherichia coli) | secreted expression of the heat-labile enterotoxin subunit B (LTB) using the native bacterial DNA sequence (SF) [27] | 8 mg·l-1 culture |
LTB (Escherichia coli) | intracellular expression of a LTB and a viral antigen fusion protein using the native bacterial DNA sequence (SF) [27] | N.D. |