Table 4 ¹³C labeling patterns of amino acids in cell hydrolysates of Saccharomyces cerevisiae cultivatedon [1-¹³C] glucose in chemostat under aerobic glucose-limited conditions at different growth rates.

Alanine (m/z 260, C_1–3)

calc^a

0.647

0.328

0.596

0.380

0.539

0.435

exp^b

0.652

0.331

0.597

0.381

0.543

0.442

Alanine (m/z 232, C_2–3)

calc

0.671

0.316

0.618

0.372

0.561

0.427

exp

0.666

0.325

0.615

0.374

0.549

0.442

Glycine (m/z 246, C₂)

calc

0.922

0.074

0.932

0.065

0.964

0.035

exp

0.922

0.074

0.931

0.064

0.963

0.036

Valine (m/z 186, C_2–5)

calc

0.450

0.424

0.117

0.365

0.457

0.165

0.308

0.471

0.203

exp

0.442

0.424

0.116

0.361

0.451

0.165

0.301

0.480

0.203

Valine (m/z 260, C_2–5)

calc

0.450

0.424

0.117

0.365

0.457

0.165

0.308

0.471

0.203

exp

0.442

0.428

0.120

0.364

0.457

0.170

0.300

0.475

0.206

Valine (m/z 288, C_2–5)

calc

0.434

0.425

0.128

0.346

0.451

0.179

0.288

0.464

0.220

exp

0.435

0.429

0.125

0.355

0.456

0.174

0.294

0.475

0.210

Serine (m/z 288, C_2–3)

calc

0.694

0.303

0.628

0.369

0.566

0.430

exp

0.693

0.300

0.624

0.366

0.569

0.422

Serine (m/z 362, C_2–3)

calc

0.694

0.303

0.628

0.369

0.566

0.430

exp

0.693

0.302

0.623

0.369

0.567

0.424

Serine (m/z 390, C_1–3)

calc

0.682

0.311

0.611

0.375

0.560

0.431

exp

0.682

0.308

0.617

0.370

0.564

0.428

Threonine (m/z 376, C_2–4)

calc

0.541

0.381

0.526

0.409

0.515

0.440

exp

0.540

0.387

0.521

0.409

0.504

0.455

Threonine (m/z 404, C_1–4)

calc

0.496

0.396

0.504

0.413

0.488

0.444

exp

0.500

0.398

0.505

0.411

0.496

0.455

Aspartate (m/z 418, C_1–4)

calc

0.496

0.396

0.504

0.413

0.488

0.444

exp

0.502

0.399

0.506

0.415

0.499

0.454

Arginine (m/z 414, C_1–5)

calc

0.302

0.417

0.219

0.239

0.410

0.261

0.263

0.450

0.241

exp

0.309

0.415

0.218

0.245

0.406

0.260

0.263

0.450

0.244

Phenylalanine (m/z 336, C_1–9)

calc

0.379

0.429

0.166

0.304

0.437

0.212

0.240

0.442

0.262

exp

0.371

0.417

0.166

0.305

0.431

0.212

0.238

0.437

0.263

Phenylalanine (m/z 302, C_1–2)

calc

0.973

0.961

0.979

exp

0.957

0.952

0.975

Tyrosine (m/z 466, C_1–9)

calc

0.379

0.429

0.166

0.304

0.437

0.212

0.240

0.442

0.262

exp

0.379

0.421

0.165

0.304

0.432

0.213

0.242

0.440

0.250

Tyrosine (m/z 302, C_1–2)

calc

0.973

0.961

0.979

exp

0.972

0.964

0.966

Glutamate (m/z 432, C_1–5)

calc

0.352

0.427

0.184

0.278

0.431

0.233

0.276

0.458

0.230

exp

0.353

0.429

0.184

0.278

0.432

0.233

0.279

0.467

0.228