Figure 2

Immunoblot analysis of protein production using anti-Tdk serum (panels A and C-G) or anti-NilC serum (panel B). E. coli KY895 (panels A, B, F, G), BL21 Star™ (DE3) (panel C), KY895 clpP (panel D), or BL21 (DE3) (panel E) carrying plasmids pTara and pET-28a(+) (vector-only; lane 1), pETSTdk or pETSNilC (short-form; lane 2), pETLTdk or pETLNilC (long-form; lane 3), pETMAGPTdk or pETMAGPNilC (D2A change; lane 4), pETMDAPTdk or pETMDAPNilC (G3A change; lane 5) or pETMDGATdk or pETMDGANilC (P4A change; lane 6) was grown overnight in LB/kan/cam and separated cellular proteins were subjected to immunoblot analysis with the indicated antiserum. Arabinose (0.2%) was added to the culture medium for the strains in panels F and G, and IPTG (0.2 mM) was added to the culture medium for the strains in panel G. Similar volumes of cultures were used in each lane but cultures were not normalized for optical density or overall protein concentrations.