Figure 1
From: Use of pIVEX plasmids for protein overproduction in Escherichia coli
Comparison of MalE overproduction between in vitro and in vivo expression systems. RTS500 E. coli HY extracts (A) and BL21(DE3)/pDIA17 whole cell lysates were separated on 12% SDS-polyacrylamide gel electrophoresis followed by staining with Coomassie blue. Lanes 1, molecular weight marker; lanes 2, pIVEX2.4d; lanes 3, pIV2.1ME; lanes 4, pIV2.2ME; lanes 5, pIV2.3ME; lanes 6, pIV2.4ME.