Figure 5
From: Expression of yeast deubiquitination enzyme UBP1 analogues in E. coli
Expression level of UBP1 analogue with Q754L mutation (UBPQL) as revealed by SDS electrophoresis in 12% polyacrylamide gel. 1 – molecular mass marker (kDa); 2 and 3 – lysates E. coli BLD21(DE3) bacteria transformed with pT7UPQL, cultured, not induced and induced with IPTG, respectively. The UBPQL protease and ubiquitin bands are marked with arrows.