Figure 1

Nucleotide and amino-acid sequences of the yeast UBP1 gene and UBP1 protease [2]. A) The nucleotide residues are numbered on the left, and amino acid residues are numbered on the right of the figure. The underlined AGA or AGG arginine and TTA leucine codons were changed for CGC or CGT and CTG triplets, respectively. The CAG triplet (shown in bold) at positions 2260–2262 was changed to CTG one (Q754L mutation). The amino-acids forming active centre is shown in italics [4]. For the construction of UBI::UBPDQL or UBI::UBPD2QL coding sequences the first 162 bp or 294 bp which were removed are overlined or underlined, respectively. The 3'part of the UBP1 gene (positions: 2074–2430) was used to construct the S protein, the truncated hybrid protein which was used to determine the UBP1 variants' activity. B) Schematic structure of UBI::UBP1 construction variants. – ubiquitin, □ – UBP1, – 6xHis.