Table 2 Membrane functional changes as a function of growth rate and cultivation technique
From: Characterisation of the Escherichia coli membrane structure and function during fedbatch cultivation
FUNCTION 2 | Continuous cultivation | Fed-batch cultivation | Comment | ||||
|---|---|---|---|---|---|---|---|
Parameter | Low μ | Min/Max | High μ | Low μ | Min/Max | High μ |  |
Total protein production (per CDW) | R | High | High | R | High | High | Yield drops from the theoretical 0.5 to approx. 0.4 |
Specific protein production (per tot prod.) | R | High | High. | R | High | High | Model substance: periplasmic β-lactamase |
Cell lysis (%) | 8 | 1 | 1 | 1 | <1 | <1 | Â |
Effect of sonication (% release of specific protein) | 56 | 78 | 73 | 57 | 80 | 85 | Result of 1 min sonication |
Endotoxin release (% of CDW) | 1 | 1 | 1 | <1 | <1 | <1 | Â |
Periplasmic leakage | 1.5 | 19 | 7 | 10 | 10 | 9 | Â |