Figure 3

In vitro and in vivo fates for MalE31. A- The maltose-bound state of MalE31. The helix α1 and strand βB of the N-domain (MalE31), colored in magenta, and the helix αVII and strand βJ of the C-domain (MalE219), colored in blue, are located in the crystal structure of MalE. For both turns, the Gly residue is shown in yellow. The bound maltose substrate in black is located in a cleft between both domains. B- Periplasmic inclusion bodies of MalE31. Transmission electron micrograph of ultrathin sections of E. coli overproducing malE31 at 30°C. The black arrows indicate the position of periplasmic inclusion bodies.