Figure 2
From: Identification of pathogenic microbial cells and spores by electrochemical detection on a biochip
Accessibility of B. cereus cell lysate obtained by two-cycle French Press disintegration for the electric chip assay of the hblC gene. The lysate was heat treated (95°C/10 min) and solid particles were removed by centrifugation (5000 g/10 min). The hblC DNA targets were detected in an equivalent of 1010, 109, and 108 bacterial cells, using 2 × 107 capturing beads and 10 nM HblC D2 probe (1 h hybridization at 40°C, 1 h enzyme binding, and 2 h enzymatic reaction at 30°C). Control I: capturing beads and target without detection probe; Control II: capturing beads and detection probe without target; Control III: capturing beads without target and detection probe.