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Table 4 Differentially expressed genes of the core metabolism and ATP synthesis for which significant values were obtained.

From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Metabolic Pathway

Common Gene Name

pH 5

pH 3

 

Core metabolism

CDC19

â–¼

â–¼

Pyruvate kinase

 

ENO1

â–¼

â–¼

Enolase

 

FBA1

â–¼

â–¼

Fructose 1, 6-bisphosphate adolase

 

FBP1

â–²

n.s.

Fructose-1, 6-bisphosphatase

 

GPM1

—

â–¼

phosphoglycerat mutase

 

PCK1

— tend.

â–¼

Phosphoenolpyruvat carboxylkinase

 

PDB1

â–¼

â–¼

Pyruvate dehydrogenase (beta subunit)

 

PDC1

â–²

n.s.

Pyruvat decarboxylase

 

PDC5

â–²

?

Pyruvat decarboxylase

 

TAL1

â–¼

â–¼

Transaldolase

 

TDH1

â–¼

â–¼

Glyceraldehyde-3-phosphate dehydrogenase

 

TDH2

â–¼

â–¼

Glyceraldehyde-3-phosphate dehydrogenase

 

TDH3

â–¼

â–¼

Glyceraldehyde-3-phosphate dehydrogenase

 

TKL1

â–²

â–²

Transketolase 1

 

FDH1

â–²

â–²

protein with similarity to formate dehydrogenase

 

FDH2

â–²

â–²

Formate dehydrogenase

 

SFA1

â–²

â–²

formaldehyde dehydrogenase

ATP synthesis

IDP1

â–¼

â–¼

isocitrate dehydrogenase

 

IDP2

â–¼

n.s.

isocitrate dehydrogenase

 

YJL045W

â–¼

n.s.

succinate dehydrogenase (ubiquinone) activity

  1. A 1.5 fold change in expression was regarded as significant difference. (▲) upregulated upon shift from glycerol to methanol, (—) expression unchanged upon shift from glycerol to methanol, (▼) downregulated upon shift from glycerol to methanol, (n.s.) no significant values were obtained, (tend.) signifies that one of the two microarrays did not allow to obtain a significant value, or that one of the values is changed less than 1.5 fold, a question mark (?) signifies that both values for this experiment are significant but show the opposite change of expression, the result is therefore not interpretable.