Figure 5From: Characterization of the iron-regulated desA promoter of Streptomyces pilosus as a system for controlled gene expression in actinomycetesIron regulation of expression of the reporter α-amylase gene coupled to the desA promoter in a multicopy transformant (pUL99DA) (panel A) and a low-copy number transformant (pUL99DA) (panel B) expressed as volumetric enzyme activity (panels A and B) or as specific activity (panel C). ○, ●, pUL99DA; ▼, ▽, pUL42DA. Closed symbols, control cultures in LS medium; open symbols, cultures induced with the iron-complexing agent 2,2'-dypirydyl added at time 0 (inclined arrows).Back to article page