Table 2 Fed-batch production of Adh with IPTG and concentrated whey permeate/glycerol mixture. The results were calculated from samples at 18 hours after induction.
From: Cheese whey-induced high-cell-density production of recombinant proteins in Escherichia coli
Inducer | Carbon source | Digestion of carbon source | Cell wet weight | Yield |
|---|---|---|---|---|
0.5 mM IPTG | Glucose | 100% | 200 g l-1 | 7000 U g-1 |
Lactose (whey) | Glycerol /lactose (whey) | 100% / 35% | 203 g l-1 | 7200 U g-1 |