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Figure 4 | Microbial Cell Factories

Figure 4

From: Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae

Figure 4

Using LOB7-rFc and V9 antibody for Immunocytochemistry. The cell line ASF-2 were stained with LOB7 scFv-rFc (A, B) and V9 (D, E) and detected with Goat-anti-Rabbit Alexa Fluor 488 (green) and Goat-anti-Mouse Alexa Fluor 546 (red) respectively. Negative controls (C, F) were done leaving out primary antibodies. The cell nuclei were stained with DAPI (blue). Both LOB7 and V9 detect vimentin, a cytoplasmic intermediate filament. Both give excellent staining. (Scale bar, 20 μm).

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Microbial Cell Factories

ISSN: 1475-2859

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