Table 4 Expression profile of the genes belonging to PHA biosynthesis and central metabolic pathways in the metabolically engineered P. putida strains compared to their parental strains
Gene name | Locus tag | Description | Fold change§ | |
|---|---|---|---|---|
Δgcd-acoA | KT2440-acoA | |||
PHA synthesis | ||||
phaI | PP5008 | PHA granule-associated | 0.7 | 0.9 |
phaF | PP5007 | PHA granule-associated | -0.3 | 0.2 |
phaC1 | PP5003 | PHA polymerase | -0.2 | 0.7 |
phaC2 | PP5005 | PHA polymerase | -0.6 | -0.2 |
phaZ | PP5004 | PHA depolymerase | 0.3 | 0.5 |
phaD | PP5006 | Transcriptional regulator | -0.4 | 0.3 |
phaG | PP1408 | Acyl-transferase | 1.5 | -0.1 |
Transporters | ||||
oprB-1 | PP1019 | Porin | -0.7 | -1.0 |
oprB-2 | PP1445 | Porin | -0.5 | -0.6 |
Glycolysis/gluconeogenesis | ||||
glk | PP1011 | Glucokinase | 0.2 | -0.2 |
pgi | PP1808 | Glucose-6-phosphate isomerase | -0.5 | 0.0 |
fbp | PP5040 | Fructose-1,6-bisphosphatase | 0.6 | 0.4 |
fda | PP4960 | Fructose-1,6-bisphosphate aldolase | 0.9 | 0.1 |
tpiA | PP4715 | Triosephosphate isomerase | 0.8 | 0.6 |
gap1 | PP1009 | GAP dehydrogenase, type I | -1.2 | -0.4 |
gap2 | PP2149 | GAP dehydrogenase, type II | 0.2 | 0.3 |
pgk | PP4963 | Phosphoglycerate kinase | 0.2 | 0.1 |
pgm | PP5056 | Phosphoglyceromutase | 0.4 | 0.1 |
eno | PP1612 | Phosphopyruvate hydratase | 0.3 | 0.0 |
pyk | PP1362 | Pyruvate kinase | -0.3 | 0.4 |
Pentose phosphate pathways | ||||
zwf1 | PP1022 | G6P dehydrogenase | 0.9 | 0.2 |
zwf2 | PP4042 | 0.1 | -0.3 | |
zwf3 | PP5351 | 0.2 | 0.4 | |
pgl | PP1023 | 6-phosphogluconate dehydrogenase | 0.5 | -0.9 |
gnd | PP4043 | 6-phosphoglucolacto dehydrogenase | -0.6 | -0.1 |
gnuK | PP3416 | Carbohydrate kinase | -1.1 | -0.6 |
kguK | PP3378 | Dehydroglucokinase | 0.45 | -0.4 |
kguD | PP3376 | 2-Ketogluconate 6-phosphate reductase | -0.7 | -0.5 |
rpiA | PP5150 | Ribose-5-phosphate isomerase A | 0.3 | 0.0 |
rpe | PP0415 | Ribulose-phosphate 3-epimerase | -0.5 | -0.1 |
tktA | PP4965 | Transketolase | 0.3 | 0.7 |
tal | PP2168 | Transaldolase B | 0.4 | 0.3 |
Entner-Doudoroff pathway | ||||
edd | PP1010 | 6-Phosphogluconate dehydratase | -0.3 | -0.3 |
eda | PP1024 | KDPG aldolase | 0.0 | -0.5 |
Pyruvate metabolism | ||||
acoA | PP0555 | Pyruvate dehydrogenase | 12.4 | 5.3 |
acoB | PP0554 | Pyruvate dehydrogenase | 1.5 | 1.0 |
acoC | PP0553 | Pyruvate dehydrogenase | 0.7 | 0.7 |
PP0545 | Aldehyde dehydrogenase | 0.1 | -1.0 | |
acsA | PP4487 | Acetyl-CoA synthetase | 0.8 | 1.4 |
accC-2 | PP5347 | Pyruvate carboxylase | 0.5 | 0.2 |
ppsA | PP2082 | Phosphoenolpyruvate synthase | 0.5 | 0.4 |
ppc | PP1505 | Phosphoenolpyruvate carboxylase | 0.5 | 0.4 |
TCA cycle | ||||
gltA | PP4194 | Citrate synthase | 0.7 | 0.7 |
acnA | PP2112 | Aconitate hydratase | 0.4 | -0.4 |
acnB | PP2339 | Aconitate hydratase | 0.7 | 0.3 |
icd | PP4011 | Isocitrate dehydrogenase | 0.3 | -0.5 |
sucA | PP4189 | 2-Oxoglutarate dehydrogenase | -0.7 | -0.2 |
sucD | PP4185 | Succinyl-CoA synthetase sub alpha | -0.1 | -0.3 |
sucC | PP4186 | Succinyl-CoA synthetase sub beta | 0.2 | 0.3 |
sdhA | PP4191 | Succinate dehydrogenase | 0.3 | 0.4 |
fumC | PP0944 | Fumarate hydratase | 0.5 | -1.1 |
mdh | PP0654 | Malate dehydrogenase | -0.4 | -1.3 |
Glyoxylate shunt | ||||
aceA | PP4116 | Isocitrate lyase | 2.9 | 1.3 |
glcB | PP0356 | Malate synthase | 0.8 | 0.8 |