Biocatalytic anti-Prelog reduction of prochiral ketones with whole cells of Acetobacter pasteurianus GIM1.158

Table 3 Biocatalytic anti-Prelog stereoselective reduction of various prochiral carbobyl compounds with Acetobacter pasteurianus GIM1.158 cells

Prochiral ketones	V₀(×10⁻¹ μ mol/min)	Yield (%)	e.e..(%)	Config.
2-Pentanone	9.78	81.5	92.0	R
Pinacolone	11.3	94.5	95.2	R
4'-Chloroacetophenone	7.3	60.4	>99.9	R
4'-Methyl-acetophenone	11.9	99.5	99.6	R
4'-Hydroxyacetophenone	11.7	97.8	97.1	R
3-Chloropropiophenone	5.7	94.9	99. 7	S
Ethyl 4-chloroacetoacetate	14.9	74.4	97.3	S
Ethyl acetoacetate	17.2	85.8	98.7	R
Methyl acetoacetate	18.6	92.9	97.2	R

Reaction conditions: 2 mL TEA-HCl buffer (50 mmol/L, pH 5.0), 25 mg/mL wet cells, 5 mmol/L various prochiral ketones, 500 mmol/L isopropanol, 35°C, 120 r/min, reaction time 2 h.

ISSN: 1475-2859