Figure 5
From: Steroid conversion with CYP106A2 – production of pharmaceutically interesting DHEA metabolites
Discontinuous feed of DHEA to B. megaterium ATCC13368. B. megaterium ATCC13368 cultures were incubated at 30°C and 150 rpm in broth. After 16 h cultivation 400 μM DHEA was added (0 h) 5 times with 3 hours break between the individual additions. After the last addition (reaching 2000 μM DHEA in total) the cultures were incubated for additional 12 h. DHEA concentration in the cultures was calculated using TLC analysis and the software ImageLab™ (BIO-RAD, Germany).