Figure 8

In vivo antigen presentation. (A) The frequency of OTII CD4+ T cells isolated from the mesenteric lymph nodes of mice that were administered orally (1) PBS, (2) Salmonella SL7207, (3) SL7207pUC18-OVA or (4) SL7207pZS1205-4 [(OVA-CD4)x4]. The cells were gated on CD4+ CD3+ live lymphocytes and the transferred T cells were identified using the congenic marker CD45.1. (B) The expression of the surface markers CD62L, CD44 and CD25 on the gated OTII T cells isolated from the four groups described above; PBS (grey area), Salmonella SL7207 (black line), SL7207pUC18-OVA (blue line) and SL7207pZS1205-4 [(OVA-CD4)x4] (red line). (C) The total numbers of OTII T cells recovered from the mesenteric lymph nodes of the four groups of mice described above. The data shown are representative of three independent experiments.