Table 2 Primers used in this work
From: A bacteriocin gene cluster able to enhance plasmid maintenance in Lactococcus lactis
Primer | Sequence 5’-3’ (restriction site) | Position |
|---|---|---|
orf4-F | CCGCTGGAATTC TCAAACG (Eco RI) | 5’ orf4 in pBL1 |
orf4-R | CACCGGCACGTTGTG TATCC (Dra III) | 3’ lcn972 |
ery5 | GAAGATCTAGATATAATGGGAGATAAGACGG | 5’ ery in pIL252 |
SPCf | GCGGATCCGATATCGAATTC TGTAGACCCTCCG (Eco RI) | 5’ Δorf4 in pDorf4 |
SPCr | CGGAATTC CCGGGCTCGAGTCTAGAG (Eco RI) | 3’ orf3 in pDorf4 |
lcn2 | CTTGTTAAAAGAATTCGCTGCAAACC | 3’ orf2 in pBL1 |
eryrepF-F | GCGCTTAGAATCGCTTTAGG | intergenic ery-repF |
repE-F | GATTGGCGGTCGTGGTGTTG | 5’ repE |
repE-R | GTCACATAGACTGGCGTTTC | repE |
repE2-R | TAGGGCGTTCTGCTAGCTTG | 3’ repE |
201 g-F | GAGCTAAAGAGGTCCCTAGCGCTTAGAATC | 5’ repF |
201 g-R | GAAGTCAGAACAACACCACGACCGCCAATC | intergenic ery-repF |
402 g-F | GGCGGTCGTGGTGTTGTTCTGACTTCC | repE |
402 g-R | TTCCTGATTCGCCTTCAGTACCTTCAGC | repE |