Table 1 Enzymatic activities identified by screening the ikaite strain collection
Activity | Isolates | No. of 16S sequences | Closest relative (16S rDNA % identity) | Class |
|---|---|---|---|---|
Phosphatase | 102 | |||
α-Galactosidase | 75 | |||
Protease | 73 | |||
α-Amylase | 57 | |||
β-Galactosidase | 25 | |||
β-Glucanase | 24 | |||
Cellulase | 1 | |||
β-Xylanase | 1 | |||
β-Mannanase | 0 | |||
Total | 358 | |||
Groups of activity | Total no. | |||
α-Galactosidase | 45 | 3 | Loktanella vestfoldensis (98-100%) | α-Proteobacteria |
Phosphatase + protease | 32 | 5 | Arsukibacterium ikkense (98-100%) | γ-Proteobacteria |
Phosphatase | 29 | 4 | Pseudomonas sabulinigri (99%) | γ-Proteobacteria |
1 | Loktanella vestfoldensis (100%) | α-Proteobacteria | ||
1 | Stenotrophomonas rhizophila (100%) | γ-Proteobacteria | ||
1 | Arsukibacterium ikkense (100%) | γ-Proteobacteria | ||
1 | Idiomarina fontislapidosi (95%) | γ-Proteobacteria | ||
α-Galactosidase + β-galactosidase | 20 | 2 | Natronobacillus azotifigens (98%) | Bacilli |
1 | Rhodonellum psychrophilum (100%) | Bacteroidetes | ||
1 | Klebsiella pneumoniae (95%) | γ-Proteobacteria | ||
α-Amylase | 17 | 1 | Pseudomonas poae (100%) | γ-Proteobacteria |
1 | Marinovum algicola (100%) | α-Proteobacteria | ||
1 | Thermoleophilum minutum (100%) | Actinobacteria | ||
1 | Rhodobacter veldkampii (96%) | α-Proteobacteria | ||
Phosphatase + α-amylase + protease + β-glucanase | 17 | 9 | Arsukibacterium ikkense (98-100%) | γ-Proteobacteria |
Phosphatase + α-amylase + protease | 10 | 7 | Arsukibacterium ikkense (100%) | γ-Proteobacteria |
1 | Pseudomonas sabulinigri (99%) | γ-Proteobacteria | ||
Protease | 8 | 2 | Pseudomonas sabulinigri (96-97%) | γ-Proteobacteria |
1 | Aquimonas voraii (93%) | γ-Proteobacteria | ||
Phosphatase + α-amylase | 7 | 5 | Arsukibacterium ikkense (98-100%) | γ-Proteobacteria |
Phosphatase + protease + β-glucanase | 7 | 4 | Arsukibacterium ikkense (98-100%) | γ-Proteobacteria |
1 | Klebsiella variicola (97%) | γ-Proteobacteria | ||
α-Galactosidase + β-galactosidase + protease | 3 | 3 | Rhodonellum psychrophilum (96-99%) | Bacteroidetes |
α-Galactosidase + β-galactosidase + α-amylase | 2 | 2 | Rhodonellum psychrophilum (95-99%) | Bacteroidetes |
α-Galactosidase + α-amylase | 2 | 2 | Loktanella vestfoldensis (100%) | α-Proteobacteria |
α-Galactosidase + α-amylase + protease | 1 | 1 | Loktanella vestfoldensis (100%) | α-Proteobacteria |
α-Galactosidase + phosphatase | 1 | 1 | Loktanella vestfoldensis (100%) | α-Proteobacteria |
α-Galactosidase + cellulase + β-xylanase | 1 | 1 | Demequina aestuarii (98%) | Actinobacteria |
α-Amylase + protease | 1 | 1 | Arsukibacterium ikkense (100%) | γ-Proteobacteria |
Total | 203 | 65 |