Table 1 Strains and plasmid used in this study
From: Aspergillus oryzae-based cell factory for direct kojic acid production from cellulose
Strains and plasmids | Relevant features | Reference |
|---|---|---|
Escherichia coli strain | ||
NovaBlue | endA1 hsdR17(r K12-m K12+) supE44 thi-I gyrA96 relA1 lac recA1/F’[proAB + lacIq ZΔM15::Tn10(Tetr)] | Novagen |
Aspergillus oryzae strains | ||
RIB40 | Wild type | Machida et al.[11] |
NSPlD1 | niaD - sC - adeA - ΔargB::adeA - ΔligD::argB ΔpyrG::adeA | Maruyama and Kitamoto[12] |
NSPlD1/pIS1-kojR | niaD - ::pIS1-kojR [P-sodM::kojR::T-glaB] sC - adeA- ΔargB::adeA- ΔligD::argB ΔpyrG::adeA | This study |
NSPlD1/pIS1-kojR/pIS1sC-CBHI-EGI-BGLI | niaD - ::pIS1-kojR [P-sodM::kojR::T-glaB] sC- ::pIS1sC-CBHI-EGI-BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB] adeA- ΔargB::adeA- ΔligD::argB ΔpyrG::adeA | This study |
Plasmids | ||
pISI-CBHI | CBHI gene from T. reesei containing plasmid | This study |
pIS1-svaA-CBHI | Vector for expression of CBHI from T. reesei [P-svaA::CBHI::T-glaB]; niaD marker | This study |
pISI-EG | EGI (T. reesei) gene containing plasmid | This study |
pIS1-hlyA-EGI | Vector for expression of EGI from T. reesei [P-hlyA::EG::T-hlyA]; niaD marker | This study |
pIS1-BGLI | Vector for expression BGLI from A. aculeatus [P-sodM::CutL-BGL::T-glaB]; niaD marker | This study |
pIS1-CBHI-EGI-BGLI | Vector for expression of CBHI, EGI, and BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB]; niaD marker | This study |
pIS1sC-CBHI-EGI-BGLI | Vector for expression CBHI, EGI, and BGLI [P-svaA::CBHI::T-svaA, P-hlyA::EGI::T-hlyA, P-sodM::CutL-BGLI::T-glaB]; sC marker | This study |
pIS1-kojR | Vector for expression of transcription factor (AO090113000137) [P-sodM::kojR::T-glaB]; niaD marker | This study |