Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

Table 2 DNA sequence of PCR primers used for wt- taqIIRM and syn -taqIIRM genes cloning

Name	DNA sequence	Target
FsynTaq	5′-tgataatggttgcatgtactaaggagg ttgttc ATGA CCGGTGACACCTGGGTTCTGT-3′	syn-taqIIRM gene
RsynTaq	5′-acacaggaaacagaccatggaa gtcga c TA CGGCTGGTTACCGTAAACACCGTCAC-3′	syn-taqIIRM gene
Ftaq	5′-tgataatggttgcatgtactaaggagg ttgttc ATGA CCGGAGACACTTGGGTCCTCA-3′	wt-taqIIRM gene
Rtaq	5′-acacaggaaacagaccatggaa gtcgac TCA CGGTTGGTTCCCGTAGACTCCGTCC-3′	wt-taqIIRM gene
FpRZ	5′-gtcgac ttccatggtctgtttcctgtgt-3′	Linear pRZ4737 vector backbone
RpRZ	5′-cctccttagtacatgcaaccattatca-3′	Linear pRZ4737 vector backbone

The introduced BspHI and SalI restriction sites are underlined. DNA fragments complementary to the modified pRZ4737 vector are written in small letters. DNA fragments complementary to the wt-taqIIRM and syn-taqIIRM genes are in capital letters. Stop codons are in italics. The corresponding complementary regions of forward and reverse primers are marked in bold.

ISSN: 1475-2859