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Figure 6 | Microbial Cell Factories

Figure 6

From: Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

Figure 6

Temperature range of the recombinant TaqII REase activity. (A) The temperature range of syn-TaqII REase enzymatic activity. 1 μg of bacteriophage λ DNA (= 0.32 pmol recognition sites) was digested with 23 pmol (0.16 u) of syn-TaqII in the optimal buffer supplemented with 100 μM SIN for 1 h in the temperature range from 40 to 90°C. Lane M, GeneRuler™ 1 kb DNA Ladder (Thermo Fisher Scientific/Fermentas); selected bands marked); lane K, undigested λ DNA; lane 1, 40°C, lane 2; 45°C; lane 3, 50°C; lane 4, 55°C; lane 5, 60°C; lane 6, 65°C; lane 7, 70°C; lane 8, 75°C, lane 9, 80°C; lane 10, 85°C, lane 11, 90°C. (B) The dependence of relative activity of syn-TaqII on the reaction temperature.

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Microbial Cell Factories

ISSN: 1475-2859

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