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Table 1 Bioreductions of ketoisophorone (1a), α-methyl- trans -cinnamaldehyde (2a), and trans -β-methyl-β-nitrostyrene (3a), with recombinant S. cerevisiae BY4741Oye2 bearing heterologous OYE genes

From: Recombinant S. cerevisiae expressing Old Yellow Enzymes from non-conventional yeasts: an easy system for selective reduction of activated alkenes

OYE gene source 1a → 1b* 2a → 2c 3a → 3b
Time 1b (R)-1b Time 2c (S)-2c Time 3b (R)-3b
  h % e.e.% h % e.e.% h % e.e.%
C. castellii DBVPG3704 5 ≥ 99 70 24 70 ≥ 98 2 45 50
C. sake DBVPG6162 5 97 71 3 100 59 0.5 93 48
K. exigua L10 5 94 82 24 100 ≥ 98 0.5 97 30
K. lodderae DBVPG6308 5 ≥ 99 78 16 100 80 0.5 97 58
K. spencerorum DBVPG6748 5 96 65 3 100 ≥ 98 0.5 94 50
Nak. bacillisporus DBVPG6945 5 98 68 2 100 95 0.5 92 40
Nau. castellii DBVPG6298 5 93 78 2 100 85 5 92 29
S. cerevisiae BY4741 (Oye2) 5 89 80 2 100 ≥ 98 1 96 50
S. cerevisiae L12 5 86 79 2 100 72 2 95 44
  1. Data are reported as molar conversion (%). *Results from Raimondi et al. 2010. 30% of compound 2d was also observed. The experiment were performed in triplicate, the yield standard deviations (SD) were always less than 4%, and the ee% SD were always less than 3%.