Recombinant S. cerevisiae expressing Old Yellow Enzymes from non-conventional yeasts: an easy system for selective reduction of activated alkenes

Table 1 Bioreductions of ketoisophorone (1a), α-methyl- trans -cinnamaldehyde (2a), and trans -β-methyl-β-nitrostyrene (3a), with recombinant S. cerevisiae BY4741∆Oye2 bearing heterologous OYE genes

OYE gene source	1a → 1b*			2a → 2c			3a → 3b
OYE gene source	Time	1b	(R)-1b	Time	2c	(S)-2c	Time	3b	(R)-3b
	h	%	e.e.%	h	%	e.e.%	h	%	e.e.%
C. castellii DBVPG3704	5	≥ 99	70	24	70^†	≥ 98	2	45	50
C. sake DBVPG6162	5	97	71	3	100	59	0.5	93	48
K. exigua L10	5	94	82	24	100	≥ 98	0.5	97	30
K. lodderae DBVPG6308	5	≥ 99	78	16	100	80	0.5	97	58
K. spencerorum DBVPG6748	5	96	65	3	100	≥ 98	0.5	94	50
Nak. bacillisporus DBVPG6945	5	98	68	2	100	95	0.5	92	40
Nau. castellii DBVPG6298	5	93	78	2	100	85	5	92	29
S. cerevisiae BY4741 (Oye2)	5	89	80	2	100	≥ 98	1	96	50
S. cerevisiae L12	5	86	79	2	100	72	2	95	44

Data are reported as molar conversion (%). *Results from Raimondi et al. 2010. ^†30% of compound 2d was also observed. The experiment were performed in triplicate, the yield standard deviations (SD) were always less than 4%, and the ee% SD were always less than 3%.

ISSN: 1475-2859