Figure 3From: Antibiotic-free segregational plasmid stabilization in Escherichia coli owing to the knockout of triosephosphate isomerase (tpiA) Auxotrophy complementation of tpiA knockout strains. Growth of tpiA knockout strain (JW3890-2) and complemented knockout strains (JW3890-2-pFC1 and JW3890-2-pFC4) were tested in different media based on glycerol only (SGA) or complex carbon sources (LB) in the presence of kanamycin in shake flasks.Back to article page