Skip to main content

Table 1 Fab production yields by test tube culture

From: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Strain

Fab production yield (mg/l)

YDK010

11.1 ± 0.3

YDK010Δpbp1a

57.6 ± 2.3

YDK010Δpbp1b

10.6 ± 0.5

ATCC13869

6.4 ± 0.4

ATCC13869ΔcspB

8.9 ± 0.8

ATCC13869Δpbp1a

2.2 ± 0.4

ATCC13869ΔcspBΔpbp1a

34.9 ± 1.8

ATCC13869Δpbp1b

8.1 ± 1.1

ATCC13869ΔcspBΔpbp1b

7.9 ± 1.3

  1. The amount of secreted Fab in culture supernatant was quantified as described in Methods. Yields represent the average of measurements from three separate test tube cultures.
Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us