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Figure 7 | Microbial Cell Factories

Figure 7

From: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Figure 7

Cell wall-permeability barriers to the recombinant Fab(H+L) secretion by C. glutamicum . HC and LC of the Fab fragment are secreted through the cytoplasmic membrane by the Sec machinery and form a heterodimer with an intermolecular disulfide bond (S–S) in the extracellular space. There are at least two crucial permeability barriers (X) interrupting Fab secretion; the PG-arabinogalactan layer synthesized by PBP1a and the S-layer composed of CspB. These two structures also function as permeability barriers to lysozyme.

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Microbial Cell Factories

ISSN: 1475-2859

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