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Figure 4 | Microbial Cell Factories

Figure 4

From: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Figure 4

Effects of Δpbp1a and ΔcspB mutations on Fab secretion by C. glutamicum wild-type strain ATCC13869. Supernatant proteins were separated by nonreducing SDS-PAGE followed by SYPRO Orange staining. Ten microliters of supernatant mixed with an equal volume of sample buffer were loaded into each lane. Lane 1, ATCC13869/pPK4 (empty vector); lane 2, ATCC13869/pPKStrastFabHL; lane 3, ATCC13869ΔcspB/pPKStrastFabHL; lane 4, ATCC13869Δpbp1a/pPKStrastFabHL; lane 5, ATCC13869ΔcspBΔpbp1a/pPKStrastFabHL; lane 6, ATCC13869Δpbp1b/pPKStrastFabHL; lane 7, ATCC13869ΔcspBΔpbp1b/pPKStrastFabHL; M, molecular weight marker. The Δpbp1a mutation increases Fab secretion only in the presence of ΔcspB (ΔcspBΔpbp1a double mutant). The data shown represent three independent experiments that yielded similar results.

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Microbial Cell Factories

ISSN: 1475-2859

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