Skip to main content
Figure 3 | Microbial Cell Factories

Figure 3

From: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Figure 3

Effect of Δpbp1a or Δpbp1b mutation on Fab secretion by C. glutamicum YDK010 strain. Supernatant proteins were separated by non-reducing SDS-PAGE followed by SYPRO Orange staining. Ten microliters of supernatant mixed with an equal volume of sample buffer were loaded into each lane. Lane 1, YDK010/pPK4 (empty vector), lane 2, YDK010/pPKStrastFabHL; lane 3, YDK010Δpbp1a/pPKStrastFabHL; lane 4, YDK010Δpbp1b/pPKStrastFabHL; M, molecular weight marker. Mutation Δpbp1a but not Δpbp1b markedly increases Fab secretion from YDK010. The data shown represent three independent experiments that yielded similar results.

Back to article page

Microbial Cell Factories

ISSN: 1475-2859

Contact us