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Figure 2 | Microbial Cell Factories

Figure 2

From: Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum

Figure 2

Production of Fab(H+L) by C. glutamicum YDK010 carrying pPKStrastFabHL. Supernatant proteins were separated by nonreducing SDS-PAGE. Ten microliters of supernatant mixed with an equal volume of sample buffer were loaded into each lane. (a) A gel stained with SYPRO Orange; (b) Western blot of supernatant proteins probed with anti-human IgG(H+L) antibody. Lane 1, YDK010/pPK4 (empty vector); lane 2, YDK010/pPKStrastFabHL. HC, heavy chain; LC, light chain; M, molecular weight marker.

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Microbial Cell Factories

ISSN: 1475-2859

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