Figure 7
From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems
Cultivation of K. lactis GG799 for the investigation of the influence of morphological changes on the scattered light signal for online monitoring of microbial growth. (A) Online measurement of microbial growth (via scattered light) and DOT (via optodes); determination of galactose concentration in the medium from offline samples (via HPLC). Boxes FC1-6 and M1-4 indicate time points of samples for flow cytometric (C) and microscopic (D) analysis, respectively. (B) Determination of DCW and cell count (via flow cytometry) from offline samples. (C) Forward scatter histograms from flow cytometric analysis at different time points (FC1-6). (D) Microscope images of yeast cells at different time points (M1-4). Cultivation conditions: 48well FlowerPlate with optodes for DOT and pH measurement, VL = 800 μL, n = 1100 rpm, d0 = 3 mm, 30°C,YNB medium with 20 g/L galactose as substrate and inducer.