Figure 6
From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems
Characterization of the influence of red fluorescence from mCherry on the scattered light signal for online monitoring of microbial growth. (A) Dependency of the scattered light signal from mCherry fluorescence at different scattered light wavelengths. Different amounts of purified mCherry resulting in increasing fluorescence intensities were added to a suspension of non-induced E. coli cells with an OD600 = 4.9. (B) Scattered light wavelength scan of E. coli cell suspensions with varied biomass concentration (OD600). (C) Calibration curves between OD600 and scattered light intensity resulting from scattered light wavelength scan (comp. Figure 6B) at different scattered light wavelengths.