Figure 1
From: Pitfalls in optical on-line monitoring for high-throughput screening of microbial systems
Cultivation of 3 E. coli BL21 clones expressing different fluorescent proteins under non-induced (left column, open symbols) and induced conditions (right column, closed symbols) using the BioLector system. Online monitoring of microbial growth via scattered light (A,B), fluorescence intensity of the recombinant expressed fluorescent proteins (C,D), DOT (E,F) and pH value (G,H) via optodes. Note: Altered DOT scale in Figure 1F at higher values. Cultivation conditions: 48well FlowerPlate with optodes for DOT and pH measurement, VL = 800 μL, n = 1100 rpm, d0 = 3 mm, 37°C, Wilms-MOPS medium with 20 g/L glucose, induction with 0.1 mM IPTG after 6 h (indicated by dotted line).