Figure 4
From: Highly purified mussel adhesive protein to secure biosafety for in vivo applications
Assessment of purity of recombinant MAP. (A) Total carbohydrate/lipid amount determination and (B) HPLC chromatograms for protein purity determination. The data represent mean +/− standard deviation and were analyzed 2-tails student t-test (*p < 0.05, **p < 0.01, ***p < 0.005). Abbreviations: WC, whole cells; IB, isolated inclusion body containing MAP after disruption of CaCl2/EDTA-treated whole cells; X-114/114, acetic acid-extracted MAP after inclusion body washing with Triton X-114 twice. P2, second peak fraction from cation exchanger of acetic acid-extracted solution after inclusion body washing with Triton X-114 twice.