Table 2 Plasmids and oligonucleotides used
From: Production of recombinant protein by a novel oxygen-induced system in Escherichia coli
Plasmid, or primer | Genotype or sequence | Reference or source |
|---|---|---|
Plasmid | ||
pAB49 | Same as pGFPmut3.1 plus Δplac plus psoxS | This study |
pAB43 | Same as pGFPmut3.1 plus Δplac | This study |
pAB828 | Same as pGFPmut3.1 plus ::lacZ-GFPmut3.1 fusion | This study |
Primers | ||
GFPmut-A | ATGACCATGATTACGCCAAGCTAGTAGGCCTGATAGTAGACTCTAGAGGATCCCCGGGTA | |
GFPmut-B | TACCCGGGGATCCTCTAGAGTCTACTATCAGGCCTACTAGCTTGGCGTAATCATGGTCAT | |
soxS-GFP-A | TTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGTAAATCGCTTTACCTCAAGTTAACTTGAGGAATTATACTCCCCAACAGATGAATTAACGAACTGAACACTGAAAAGAGGCAGATTTATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTG | |
soxS-GFP-B | CACCCTCTCCACTGACAGAAAATTTGTGCCCATTAACATCACCATCTAATTCAACAAGAATTGGGACAACTCCAGTGAAAAGTTCTTCTCCTTTACTCATAAATCTGCCTCTTTTCAGTGTTCAGTTCGTTAATTCATCTGTTGGGGAGTATAATTCCTCAAGTTAACTTGAGGTAAAGCGATTTACTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAA |