Figure 1
From: A new fusion protein platform for quantitatively measuring activity of multiple proteases
Overexpression, purification and DAL activity assay of the fusion protein. A. Expression of the soluble fusion protein for eDAL or sDAL with a specified tag followed by tevS in E. coli. The fusion proteins from the supernatants were detected by Western blot using anti-His6 antibody. The lane 1–6 represented His6MBP-tevS-eDAL, His6MBP-tevS-sDAL, His6GST-tevS-eDAL, His6GST-tevS-sDAL, His6-tevS-eDAL and His6-tevS-sDAL. B. Purification of the fusion proteins. The fusion proteins were purified by Ni-NTA and analyzed by SDS-PAGE. All the proteins are indicated by arrows. C. The activity of the DAL as the fusion partner in the crude extracts. D. The activity of the purified fusion proteins for DAL.