Table 1 Production of shikimic acid by various recombinant strains of Bacillus subtilis
Strains | Shikimic acid*(g/L) | Changes (%) | Notes# |
|---|---|---|---|
1A474 | 1.50 ± 0.22 | 100.0 | Parent strain |
BSSA/pHCMC04 | 1.81 ± 0.18 | 120.7 | Parent strain carrying pHCMC04 |
BSSA/pSAAroA | 1.42 ± 0.20 | 94.7 | Overexpression of aroA |
BSSA/pSAAroB | 1.74 ± 0.23 | 116.0 | Overexpression of aroB |
BSSA/pSAAroC | 1.77 ± 0.15 | 118.0 | Overexpression of aroC |
BSSA/pSAAroD | 2.30 ± 0.17 | 153.3 | Overexpression of aroD |
BSSA/pSAAroA/ pDGSAAroD | 3.20 ± 0.07 | 213.3 | Overexpression of aroD plus aroA |
BSSA/pSAAroB/pDGSAAroD | 2.99 ± 0.05 | 199.3 | Overexpression of aroD plus aroB |
BSSA/pSAAroC/pDGSAAroD | 2.90 ± 0.03 | 199.3 | Overexpression of aroD plus aroC |
BSSA/pSAAroD pDGSAAroD | 2.91 ± 0.04 | 194.0 | Overexpression of aro D on two plasmids |
BSSA(Ωtkt::pSATkt) | 3.11 ± 0.03 | 207.3 | Overexpression of tkt in BSSA/pSAAroA/pDGSAAroD |
BSSA(Ωpyk::pSAPyk) | 3.46 ± 0.04 | 230.7 | Knockout of pyk in BSSA/pSAAroA/pDGSAAroD |