Table 3 Bacterial strains and plasmids used in this study

E. coli

DH5α

Competent cells

Synechocystis

WT

WT Synechocystis sp PCC 6803, glucose tolerant

kan^S, suc^R

H. Pakrasi (Washington University, St. Louis)

lux ^prim

luxAB operon, interrupted by nptI-sacB cassette, replacing slr0168 ORF

kan^R, suc^S

This study

lux ^sec

Intact luxAB operon replacing slr0168 ORF

kan^S, suc^R

This study

psaA ^prim

At psaA gene, interrupted by nptI-sacB cassette, replacing endogenous psaA

kan^R, suc^S, heterotroph

This study

psaA ^sec

Intact At psaA gene replacing endogenous psaA

kan^S, suc^R, photoautotroph

This study

Plasmids

pGEM-T Easy

Backbone for pDSpsaA

amp^R

Promega, Madison, WI

pRL250

nptI-sacB double selection cassette, sacB gene from Bacillus subtilis

kan^R, suc^S

P. Wolk (Michigan State University)

pICH69822

Destination vector for Golden Gate cloning

kan^R

E. Weber (Icon Genetics GmbH, Halle)

pRL1063a

luxAB operon from Vibrio fischeri

Sm^R

P. Wolk (Michigan State University)

pDSlux

pICH69822 with nptI-sacB cassette from pRL250, luxAB operon from pRL1063a, Syn psbA2 promoter and slr0168 flanking regions

kan^R, suc^S

This study

pDSpsaA

pICH69822 with nptI-sacB cassette from pRL250, At psaA gene and Syn psbA2 promoter and slr0168 flanking regions

kan^R, suc^S

This study