Figure 3

Mass transfer coefficient and culture apparent viscosity assessment. Batch 1 was scaled-up and run at DO 12.5% at the large (10,000 L) scale bioreactor. Run 5 and 7 were consistency runs at DO 12.5% at the 10 L scale bioreactor. (A) Mass transfer coefficient (k _L a) comparison between two scales. (B) Apparent viscosity comparison. The apparent viscosity for the 10,000 L scale Batch 2 and one parallel at 10 L scale run at DO 12.5% was measured at 750 s⁻¹ shear rate as described in “Methods”.