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Table 4 Average expression data of upregulated genes during EXP/STA2 comparison based on resultant FDR value from the RP analysis equal or less to 0.05 in compared growth phases during SA production cultures

From: Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium

Gene

Locus

Protein namea

Cellular function GOb

Average expression

ykfE

b0220

(ivy) Protein: inhibitor of vertebrate C-type lysozyme

Amino acid metabolism

29.2978

cysK

b2414

Enzyme: cysteine synthase A

Amino acid metabolism

83.0796

gcvH

b2904

Polypeptide: glycine cleavage system H protein

Amino acid metabolism

30.4955

gcvP

b2903

Enzyme: glycine decarboxylase

Amino acid metabolism

33.3045

gcvT

b2905

Enzyme: aminomethyltransferase

Amino acid metabolism

38.3509

gdhA

b1761

Enzyme: glutamate dehydrogenase

Amino acid metabolism

20.9785

glyA

b2551

Enzyme: serine hydroxymethyltransferase

Amino acid metabolism

23.7380

tnaA

_b3708

L-cysteine desulfhydrase/tryptophanase

Amino acid metabolism

139.6125

tnaL

b3707

tna operon leader peptide

Amino acid metabolism

62.9786

ompT

b0565

Enzyme: OM protease VII (OM protein 3b)

Catalytic activity

35.7201

fimC

b4316

Polypeptide: periplasmic chaperone, required for type 1 fimbriae

Cell envelope

63.3469

fimD

b4317

Polypeptide: OM protein; export and assembly of type 1 fimbriae

Cell envelope

25.6637

nlpA

b3661

Polypeptide: lipoprotein-28

Cell envelope

48.3194

ydiQ

b1697

Polypeptide: putative subunit of YdiQ-YdiR flavoprotein

Electron carrier activity

29.9950

nrdA

b2234

Protein: ribonucleoside diphosphate reductase 1, α subunit dimer

Nucleotide/nucleoside metabolism

37.6063

yciW

b1287

Polypeptide: predicted oxidoreductasePolypeptide: predicted oxidoreductase

Out of GOs

116.1438

yieI

b3716

(cbrB) Polypeptide: predicted IM protein

Out of GOs

31.9090

yeeN

b1983

Polypeptide: conserved protein

Regulatory

22.0828

yhiE

b3512

(gadE) Polypeptide: GadE DNA-binding transcriptional activator

Regulatory

46.2077

yhiW

b3515

(gadW) Polypeptide: GadW DNA-binding transcriptional dual regulator

Regulatory

37.7545

hdeA

b3510

Protein: HdeA dimer, inactive form of acid-resistance protein

Stress response

32.4174

hdeB

b3509

Polypeptide: acid stress chaperone

Stress response

56.2185

b0023

b0023

Polypeptide: 30S ribosomal subunit protein S20

Structural molecule activity

45.8728

cysC

b2750

Enzyme: adenylylsulfate kinase

Sulfur metabolism

52.8463

cysD

cysD

Polypeptide: CysD

Sulfur metabolism

236.2573

cysH

b2762

Enzyme: 3′-phospho-adenylylsulfate reductase

Sulfur metabolism

194.5029

cysJ

b2764

Enzyme: sulfite reductase, flavoprotein subunit complex

Sulfur metabolism

62.3545

ydjN

b1729

Polypeptide: predicted transporter

Transport

43.4944

dppB

b3543

Polypeptide: dipeptide ABC transporter - putative membrane subunit

Transport

23.0830

dppF

b3540

Polypeptide: dipeptide ABC transporter - putative ABC binding subunit

Transport

32.6107

fecA

b4291

Polypeptide: ferric citrate OMP FecA

Transport

23.6130

malE

b4034

Maltose ABC transporter - periplasmic binding protein

Transport

230.4651

ompC

b2215

Transporter: OMP C

Transport

61.2832

oppB

b1244

Polypeptide: murein tripeptide ABC transporter/peptide ABC transporter - putative membrane subunit

Transport

44.3550

proV

b2677

Polypeptide: glycine betaine/proline ABC transporter - ATP binding subunit

Transport

34.7488

proW

b2678

Polypeptide: glycine betaine/proline ABC transporter - membrane subunitPolypeptide: glycine betaine/proline ABC transporter - membrane subunit

Transport

35.6683

proX

b2679

Polypeptide: glycine betaine/proline ABC transporter - periplasmic binding protein

Transport

48.2957

  1. aRetrieved from EcoCyc database, bBiological functions were assigned according to the EcoCyc database for E. coli strain MG1655 and broadly grouped on the basis of GO terms [17].