Production of shikimic acid from Escherichia coli through chemically inducible chromosomal evolution and cofactor metabolic engineering

Table 1 Shikimic acid (SA) production by different strains

Stain	OD₆₀₀	SA concentration (g/L)	Yield (mol SA/mol glucose)
BW25113 (ΔaroK ΔaroL)	7.18 ± 0.95	0.15 ± 0.04	0.05
BW25113 (ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1)	5.82 ± 0.04	0.23 ± 0.02	0.08
BW25113 (ΔaroK ΔaroL, P _pps ::P _lacQ1 , P _csrB ::P _lacQ1 , P _T5 -pps, P _T5 -csrB)	5.82 ± 0.57	0.26 ± 0.02	0.09
SA110	5.91 ± 1.34	1.34 ± 0.15	0.21
SA112	5.85 ± 0.46	1.70 ± 0.01	0.25

ISSN: 1475-2859