Metabolic engineering of Pseudomonas sp. strain VLB120 as platform biocatalyst for the production of isobutyric acid and other secondary metabolites

Table 1 Influence of yeast extract on product titers of isobutyric acid, isobutanol and 3-methyl-1-butanol

	Isobutyric acid [μM]	Isobutanol [μM]	3-methyl-1-butanol [μM]
M9*	5.4 ± 1.3	43.1 ± 0.4	50.0 ± 1.4
M9* + 5 g L^-1 yeast extract	56.4 ± 1.5	72.1 ± 2.9	236.5 ± 0.4

Cultivations (72 h) were done in M9* medium with 20 g L^-1 glucose in sealed shake flasks at 30°C with and without 5 g L^-1 yeast extract using Pseudomonas sp. strain VLB120 pCOM10-kivd. kivd overexpression was induced with 0.05% (v/v) DCPK at early exponential phase. In cultures of Pseudomonas sp. strain pCOM10 without overexpression of kivd none of the listed products were detectable. Errors indicate standard deviations (n = 2).

ISSN: 1475-2859