Figure 2

Growing cell 2-ketoisovalerate biotransformations. Comparison of Ps. sp. strain VLB120 pCOM10-kivd (A) and Ps. sp. strain VLB120Δbkd pCOM10-kivd (B). Cultivations were performed in M9* pH 7.0, 5 g L^-1 glucose in sealed shake flasks at 30°C. Cells were induced at early exponential phase with 0.05% (v/v) DCPK and 25 mM 2-KIV was added. ▼: 2-ketoisovalerate, □: isobutyric acid, ○: isobutanol. Two independent fermentations were performed, showing comparable results.