Figure 2
Expression of lipase fusion protein, expression and surface display of foldase fusion protein. A: SDS-PAGE of the outer membrane protein preparation of E. coli BL21(DE3)pAT-LipBc. Lane 1 shows an outer membrane preparation of E. coli BL21(DE3), used as a control. Lanes 2 and 3 show outer membrane preparations of E. coli BL21(DE3)pAT-LipBc. B: SDS-PAGE of the outer membrane protein preparation of E. coli BL21(DE3)pAT-FoldBc. Molecular weight markers are indicated on the left hand side. M: protein marker; IPTG: protein expression was induced by adding IPTG (final concentration: 1Â mM); Proteinase K: whole cells were treated with Proteinase K; concentrations are given in mg mL-1. The lipase and foldase fusion proteins are indicated by using black arrows. OmpA/OmpF: native E. coli outer membrane proteins are also indicated by a black arrow.