Table 2 Strains and plasmids used in this study

Escherichia coli DH5α

supE 44, △lac U169 (θ80lacZ△M15) endA1, recA1, hsdR17, thi-1 l − gyrA96, relA1; gene cloning

Transgen, China

Escherichia coli ET12567

dam, dcm, hsdM, hsdS, hsdR, cat, tet; donor for the conjugal transfer

[35]

Pseudoalteromonas sp. SM9913

Wild type

This work

pUZ8002

tra, neo, RP4; a derivative of RK2 with a mutation in oriT

[36]

pKNG101

R6K-derived suicide plasmid containing Str and sacB; template for oriT and sacB amplification

[37]

pSM429

Cryptic plasmid of Pseudoalteromonas sp. Bsi20429; template for RepA

[30]

pMG36e

Em^R, pWV01 origin, expression vector for L. lactis; template for EmR amplification

[38]