Figure 3

Verification and schematic representation of the first recombination. (A) Electrophoresis analysis of the PCR products to confirm the mutants in which the first homologous recombination making pMT insert into the chromosome of SM9913 occurred. Lanes: M, DNA marker; 1 – 6, pMT inserted mutants; 7, wild SM9913. (B) Schematic representation of the first recombination event and positions of the primers for mutation verification. UPF and DnR were the primers used for mutation verification.