Table 5 Densitometric analysis of dot blot experiments with the pellets of the adsorption reaction with wild type and cotH mutant spores and increasing amounts of LTB
LTB source | Amount of sample used | Density (OD/mm2) | Amount of LTB (ng) | Amount of LTB μg/2 x 109spore μl (total) |
|---|---|---|---|---|
Purified LTB | 25.0 ng | 122.592 | NA | NA |
12.5 ng | 66.385 | NA | NA | |
6.25 ng | 35.721 | NA | NA | |
2 μg | ||||
wt | 1 × 107 | 36.331 | 7.2 | 1.50 (75) |
5 × 106 | 22.387 | 3.8 | 1.52 (75) | |
2.5 × 106 | 14.418 | 1.2 | 1.45 (72) | |
cotH | 5 × 106 | 30.527 | 4.2 | 1.92 (96) |
2.5 × 106 | 19.157 | 2.3 | 1.86 (93) | |
1.25 × 106 | 9.564 | 1.3 | 1.79 (89) | |
10 μg | ||||
wt | 1 × 107 | 98.331 | 19.7 | 3.94 (39) |
5 × 106 | 51.387 | 8.7 | 3.48 (35) | |
2.5 × 106 | 28.418 | 3.6 | 2.88 (28) | |
cotH | 1 × 107 | 142.37 | 32 | 6.4 (64) |
5 × 106 | 97.285 | 19 | 7.6 (76) | |
2.5 × 106 | 53.644 | 10 | 8.0 (80) | |
20 μg | ||||
wt | 5 × 106 | 66.778 | 12.8 | 5.1 (25) |
25 × 106 | 42.422 | 7.5 | 6.0 (30) | |
cotH | 5 × 106 | 159.87 | 35.2 | 14.3 (71) |
2.5 × 106 | 87.56 | 17.34 | 13.8 (70) | |
1.25 × 106 | 42.422 | 9.83 | 15.0 (75) |